ABSTRACT Wasps are a diverse group of insects that possess a sting apparatus associated with a venom gland, which is used for predation and colony defense. The biochemistry of Hymenoptera venom has been evaluated in relation to allergy and immunology, and proteomics has been shown to be a powerful tool for the identification of compounds with pharmacological potential. Data on wasps venom the of genus Apoica are scarce, so the objective of the present work was to identify the venom proteins of the eusocial wasp Apoica pallens, as a first step towards further investigation of applied uses of the venom and its protein constituents. The venom proteins were separated by two-dimensional gel electrophoresis, followed by MALDI-TOF/TOF mass spectrometry. A total of 259 spots were detected, with molecular weights from 4.9 to 141 kDa. Thirty of these proteins were identified and classified into eight functional categories: allergen, enzyme, metabolism, structural, environmental response, proteoglycan, active in DNA and RNA, and unknown function. Due to the few available proteomic data for wasp venom, many proteins could not be identified, which makes studies with proteomic analysis of Hymenoptera venom even more important.

RESUMO Objetivo: Avaliar a rugosidade superficial de duas marcas de resina acrílica autopolimerizável (Clássico Dencor® e Duralay®) com dois diferentes métodos de acabamento e polimento (convencional e através do kit de acabamento e polimento da Dhpro®). Métodos: Um total de 20 corpos de prova (10 de cada marca) foi obtido através de moldes em Silicona de Condensação Zetalabor® - Zhermak. Após a polimerização, os 20 blocos de resina foram divididos ao meio e submetidos a dois tipos de acabamento e polimento, um em cada extremo. Os tipos de acabamento e polimento foram: convencional (em torno mecânico) e através do kit de acabamento e polimento da Dhpro®. Após acabados e polidos, os corpos de prova foram submetidos ao ensaio de rugosidade superficial através de um Rugosímetro e analisados por meio do teste “t” de Student. Resultados: Existe diferença significativamente estatística entre os polimentos, não relacionada à marca da resina acrílica. O polimento convencional atingiu um nivel de 0,12 µm em ambas as resinas e o polimento da DhPro® apresentou rugosidade um pouco acima de 0,2 µm. Conclusão: O polimento convencional é superior ao kit DhPro®, por atingir níveis de rugosidade mais baixos. Não existe diferença significativa entre as resinas acrílicas quanto à rugosidade superficial após os processos de acabamento e polimento.

ABSTRACT Objective : To evaluate the surface roughness of two makes of autopolymerized acrylic resin (Classic Dencor® and Duralay®) with two different methods of finishing and polishing (conventional and using the Dhpro® finishing & polishing kit). Methods : A total of 20 specimens (10 of each make) was obtained using Zetalabor®--Zhermak condensation silicone molds. After polymerization, 20 blocks of resin were divided in two and subjected to two types of finishing and polishing, one at each end. The types of finishing and polishing were as follows: conventional (lathe) and using the Dhpro® finishing & polishing kit. Once finished and polished, the specimens were subjected to surface roughness testing using a roughness meter and were analyzed via the Student’s t-test. Results : There is a statistically significant difference between the forms of polishing, unrelated to the brand of acrylic resin. Conventional polishing achieved a level of 0.12 µm for both resins and the DhPro® polishing presented roughness a little above 0.2 µm. Conclusion : Conventional polishing is superior to the DhPro® kit, as it achieves lower levels of roughness. There is no significant difference between the acrylic resins in terms of surface roughness after the finishing and polishing processes.